Journal: Applied Microbiology and Biotechnology

Article Title: Recent advances in the potential applications of luminescence-based, SPR-based, and carbon-based biosensors

doi: 10.1007/s00253-022-11901-6

Figure Lengend Snippet: Advances and potential application of biosensors in pharmaceutical sciences

Article Snippet: Electrochemical immunosensor , Zika virus (envelope protein) , 10 pM , Zika virus (strain Zika SPH2015) envelope protein (ZIKV-E) ELISA Pair Set; Sino Biological Inc. (LOD = 125 pg/mL) , Promising clinical application for early-stage diagnostics of the virus, operation time around 40 min , Kaushik et al. ( ) .

Techniques: Förster Resonance Energy Transfer, Imaging, SPR Assay, Electrochemiluminescence, MicroChIP Assay, In Vitro, Infection, Amplification, Isolation, Biomarker Assay, Sequencing

Journal: Applied Microbiology and Biotechnology

Article Title: Recent advances in the potential applications of luminescence-based, SPR-based, and carbon-based biosensors

doi: 10.1007/s00253-022-11901-6

Figure Lengend Snippet: Summarization of LOD, advances of the abovementioned biosensors, and their comparison with common methods of detection

Article Snippet: Electrochemical immunosensor , Zika virus (envelope protein) , 10 pM , Zika virus (strain Zika SPH2015) envelope protein (ZIKV-E) ELISA Pair Set; Sino Biological Inc. (LOD = 125 pg/mL) , Promising clinical application for early-stage diagnostics of the virus, operation time around 40 min , Kaushik et al. ( ) .

Techniques: Glutamate Assay, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Sequencing, Simultaneous Assay, Biomarker Assay, Fluorescence, In Situ Hybridization

Journal: NPJ Vaccines

Article Title: Immunization-induced antigen archiving enhances local memory CD8+ T cell responses following an unrelated viral infection

doi: 10.1038/s41541-024-00856-6

Figure Lengend Snippet: a Experimental schematic for b – e . C57/BL6 mice were immunized subcutaneously in the footpad and/or flank with the indicated antigens and adjuvants. b Cells were stained with CD45, PDPN, CD31 and PD-L1. Cells were gated on CD45-PDPN + CD31- for FRC and CD45-PDPN + CD31+ for LECs. Shown are examples of LEC and FRC antigen-positive cells based on PD-L1 expression (floor, MARCO LEC) and ova-AF488+ from mice 2–3 weeks after immunization with ova conjugated to Alexa-Fluor 488 (AF488) and polyI:C and αCD40. c Quantification of the frequency of LEC, BEC, and FRC in the popliteal lymph node (pLN) that are positive for the indicated antigens administered with polyI:C and αCD40 at indicated time. d Same as ( b ) except for mice were immunized with SARS-CoV-2-RBD-AF488, polyI:C, and αCD40. e Same as in ( c ) except for SARS-CoV-2-RBD and CHIKV-E2 with polyI:C and αCD40. CHIKV-E2 was repeated for 9–14 days post-vaccine (~2 weeks). Statistical analysis was done using an unpaired t -test where the p -value between naïve and indicated antigen is <0.0001. In each experiment, at least n = 2–3 mice per group were evaluated and the experiment was repeated n = 2–5 times for c – e . Shown is the representative data from one of the experiments. Error bars are mean ± standard error of the mean. ns not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Ova (10 μg) was purchased from Sigma-Aldrich (Cat No. A5503) and Chikungunya virus envelope 2 protein (8 μg) (CHIKV-E2, strain SL-CK1) was purchased from Sino Biological (Cat. No. 40440-V08B).

Techniques: Staining, Expressing

Journal: Clinical Mass Spectrometry

Article Title: Subtyping of hepatitis C virus with high resolution mass spectrometry

doi: 10.1016/j.clinms.2017.08.003

Figure Lengend Snippet: SDS-PAGE gel of deglycosylated recombinant E1 and E2 proteins.

Article Snippet: Deglycosylation and tryptic digestion of recombinant HCV envelope glycoproteins Recombinant envelope glycoproteins E1 (subtype 1b) and E2 (subtype 1a) of hepatitis C virus strains, each expressed with a polyhistidine tag at the N-terminus, were purchased from Sino Biological Inc. (Beijing, China) with predicted molecular weights of approximately 19 and 32 kDa respectively.

Techniques: SDS Page, Recombinant

Journal: Clinical Mass Spectrometry

Article Title: Subtyping of hepatitis C virus with high resolution mass spectrometry

doi: 10.1016/j.clinms.2017.08.003

Figure Lengend Snippet: Number of full-length sequences of E1 for each HCV subtype. *

Article Snippet: Deglycosylation and tryptic digestion of recombinant HCV envelope glycoproteins Recombinant envelope glycoproteins E1 (subtype 1b) and E2 (subtype 1a) of hepatitis C virus strains, each expressed with a polyhistidine tag at the N-terminus, were purchased from Sino Biological Inc. (Beijing, China) with predicted molecular weights of approximately 19 and 32 kDa respectively.

Techniques:

Journal: Clinical Mass Spectrometry

Article Title: Subtyping of hepatitis C virus with high resolution mass spectrometry

doi: 10.1016/j.clinms.2017.08.003

Figure Lengend Snippet: Subtype specific signature and indicator peptides for HCV envelope glycoprotein E1.

Article Snippet: Deglycosylation and tryptic digestion of recombinant HCV envelope glycoproteins Recombinant envelope glycoproteins E1 (subtype 1b) and E2 (subtype 1a) of hepatitis C virus strains, each expressed with a polyhistidine tag at the N-terminus, were purchased from Sino Biological Inc. (Beijing, China) with predicted molecular weights of approximately 19 and 32 kDa respectively.

Techniques: Sequencing

Journal: Clinical Mass Spectrometry

Article Title: Subtyping of hepatitis C virus with high resolution mass spectrometry

doi: 10.1016/j.clinms.2017.08.003

Figure Lengend Snippet: MALDI mass spectrum of the tryptic digest products of recombinant E1 protein of the 1b subtype.

Article Snippet: Deglycosylation and tryptic digestion of recombinant HCV envelope glycoproteins Recombinant envelope glycoproteins E1 (subtype 1b) and E2 (subtype 1a) of hepatitis C virus strains, each expressed with a polyhistidine tag at the N-terminus, were purchased from Sino Biological Inc. (Beijing, China) with predicted molecular weights of approximately 19 and 32 kDa respectively.

Techniques: Recombinant

Journal: Clinical Mass Spectrometry

Article Title: Subtyping of hepatitis C virus with high resolution mass spectrometry

doi: 10.1016/j.clinms.2017.08.003

Figure Lengend Snippet: Subtype signature and indicator peptides for HCV envelope glycoprotein E2.

Article Snippet: Deglycosylation and tryptic digestion of recombinant HCV envelope glycoproteins Recombinant envelope glycoproteins E1 (subtype 1b) and E2 (subtype 1a) of hepatitis C virus strains, each expressed with a polyhistidine tag at the N-terminus, were purchased from Sino Biological Inc. (Beijing, China) with predicted molecular weights of approximately 19 and 32 kDa respectively.

Techniques: Sequencing

Journal: Communications Biology

Article Title: Bivalent single-domain antibodies show potent mpox virus neutralization through M1R antigen

doi: 10.1038/s42003-025-08494-x

Figure Lengend Snippet: Graphical summary of anti-MPXV VHHs screening. Phage library presenting 3 × 10 9 VHHs was incubated with each biotin labeled-MPXV antigen (either A29L, A35R, B6R, E8L, H3L, or M1R), followed by washing unbound phages. After 3–4 rounds of phage display panning, antigen-bound phages were obtained. Binding of the obtained phage clones to the target antigen was validated by phage ELISA. VHH sequences were identified from the phagemids extracted from the validated clones, and monoclonal VHHs were expressed in periplasm fractions of E. coli BL21.

Article Snippet: Recombinant MPXV antigen proteins A29L (Ser21-Glu110 of QNP13733.1 in E.coli , Sino Biological 40891-V08E) , A35R (Arg58-Thr181 of EPI_ISL_13052264 in HEK293, Sino Biological 40886-V08H) , , B6R (Tyr18-His279 of QNP13760.1 in HEK293, Sino Biological 40902-V08H) , E8L (Met1-Thr275 of Q8V4Y0 in HEK293, RayBiotech 230-30232) , H3L (Met1-Pro278 of QNP13688.1 in HEK293, Sino Biological 40893-V08H1) , and M1R (Ala3-Gly183 of QNP13675.1 in HEK293, Sino Biological 40904-V07H) were used as target antigens.

Techniques: Incubation, Labeling, Binding Assay, Clone Assay, Enzyme-linked Immunosorbent Assay